Anti-ERP27 antibody [EPR12503] (ab181172) at 1/1000 dilution + Human pancreas at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Immunofluorescent staining of 4% paraformaldehyde fixed BxPC-3 cells, labeling ERP27 using ab181172 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling ERP27 using ab181172 at a 1/50 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ERP27 using ab181172 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
All lanes : Anti-C1orf163 antibody [EPR13779-15] (ab181176) at 1/1000 dilutionLane 1 : Rat pancreasLane 2 : Mouse pancreasLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase Conjugated at 1/1000 dilution