Anti-ERp72 antibody
| Name | Anti-ERp72 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab11422 |
| Prices | $379.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Bovine, Pig |
| Antigen | Synthetic peptide corresponding to Human ERp72 aa 629-645 |
| Description | Rabbit Polyclonal |
| Gene | PDIA4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab11422 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11422 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ERp72 staining in human prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11422, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Neuroligin trafficking deficiencies arising from mutations in the - Neuroligin trafficking deficiencies arising from mutations in the
De Jaco A, Lin MZ, Dubi N, Comoletti D, Miller MT, Camp S, Ellisman M, Butko MT, Tsien RY, Taylor P. J Biol Chem. 2010 Sep 10;285(37):28674-82.
Platelets release novel thiol isomerase enzymes which are recruited to the cell - Platelets release novel thiol isomerase enzymes which are recruited to the cell
Holbrook LM, Watkins NA, Simmonds AD, Jones CI, Ouwehand WH, Gibbins JM. Br J Haematol. 2010 Feb;148(4):627-37.