All lanes : Anti-ERp72 antibody [EPR14824(B)] (ab190348) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : JAR cell lysateLane 3 : Human fetal liver lysateLane 4 : MCF7 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human lung carcinoma tissue labeling ERp72 with ab190348 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.
Flow cytometry analysis of 2% paraformaldehyde-fixed MCF7 cells labeling ERp72 with ab190348 at a 1/50 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.