Anti-ESE1 antibody [EPESER1]

• Supplier: Abcam
• Catalog: ab133621
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPESER1
• Applications: WB ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant full length protein
• Description: Rabbit Monoclonal
• Gene: ELF3
• Conjugate: Unconjugated

Product images

Overlay histogram showing A431 cells stained with ab133621 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133621, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-ESE1 antibody [EPESER1] (ab133621) at 1/1000 dilutionLane 1 : Human uterus cell lysateLane 2 : Fetal lung cell lysateLane 3 : A431 cell lysateLane 4 : C6 cell lysateLane 5 : RAW 264.7 cell lysateLane 6 : PC12 cell lysateLane 7 : NIH3T3 cell lysateLane 8 : SKBR-3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunofluorescence analysis of ESE1 in A431 cells, using ab133621 antibody at a dilution of 1/250.