Anti-ESM1 antibody (ab103590) at 1/500 dilution + Human kidney tissue lysate at 50 µg
All lanes : Anti-ESM1 antibody (ab103590) at 1/500 dilutionLane 1 : ESM1 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 25 µg per lane.
ab103590 staining ESM1 in the Human HUVEC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit monoclonal (1/500) was used as the secondary antibody.See Abreview