Anti-Estrogen Related Receptor alpha antibody - N-terminal
| Name | Anti-Estrogen Related Receptor alpha antibody - N-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab137489 |
| Prices | $376.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Bovine, Dog, Pig |
| Antigen | Synthetic peptide, corresponding to a region within N terminal amino acids 1-45 of Human Estrogen Related Receptor alpha (Uniprot ID: P11474) |
| Description | Rabbit Polyclonal |
| Gene | ESRRA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab137489 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab137489 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-Estrogen Related Receptor alpha antibody - N-terminal (ab137489) at 1/500 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.