All lanes : Anti-ETS1 (phospho T38) antibody (ab59179) at 1/1000 dilutionLane 1 : Human A2058 whole cell lysateLane 2 : Human A375 whole cell lysateLysates/proteins at 50 µg per lane.SecondaryHRP-conjugated Hourse anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab59179 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59179, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59179 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59179 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.