All lanes : Anti-ETV2 antibody [EPR5229(3)] (ab181847) at 1/10000 dilutionLane 1 : HUVEC cell lysateLane 2 : HUVEC cell lysate of lent-virally expressed Human ETV2Lane 3 : Human bone marrow stromal cell lysateLane 4 : Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2developed using the ECL technique
Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells with exogenous Human ETV2 (upper panels) or without (lower panels), labeling ETV2 with ab181847 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.