All lanes : Anti-Evx1 antibody (ab15732) at 1 µg/mlLane 1 : HeLa lysateLane 2 : HeLa nuclear lysateLane 3 : Jurkat lysateLane 4 : A431 lysateLane 5 : HEK293 lysateLane 6 : HeLa lysate with Human Evx1 peptide (ab15743) at 1 µg/mlLane 7 : HeLa nuclear lysate with Human Evx1 peptide (ab15743) at 1 µg/mlLane 8 : Jurkat lysate with Human Evx1 peptide (ab15743) at 1 µg/mlLane 9 : A431 lysate with Human Evx1 peptide (ab15743) at 1 µg/mlLane 10 : HEK293 lysate with Human Evx1 peptide (ab15743) at 1 µg/mlLysates/proteins at 20 µg per lane.
ICC/IF image of ab15732 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15732, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).