Anti-EXOC6B antibody
| Name | Anti-EXOC6B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab116383 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to a region within C-terminal amino acids 766-796 of Human EXOC6B (NP_056004 |
| Description | Rabbit Polyclonal |
| Gene | EXOC6B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab116383 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab116383 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-EXOC6B antibody (ab116383) at 1/100 dilution + Mouse heart tissue lysate at 35 µg