All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/mlLane 1 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lane 2 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285)Lane 3 : Rat Ovary Tissue LysateLane 4 : Rat Colon Tissue LysateLane 5 : Rat Thymus Tissue LysateLane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.