ab122215, at 1/300 dilution, staining FAM184A in paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
All lanes : Anti-FAM184A antibody (ab122215) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : FAM184A over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cellsdeveloped using the ECL technique
Immunohistochemical analysis of Human umbilical vein endothelial cells, staining FAM184A with ab122215.Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 2% BSA for 45 minutes at 25°. Samples were incubated with primary antibody (5 µg/ml in diluent) for 1 hour at 25°C. A Cy3®-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.See Abreview