Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm and centrosome. Recommended concentration of ab122698 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-FAM45A antibody (ab122698) at 1/250 dilutionLane 1 : Vector only transfected HEK293T lysateLane 2 : FAM45A transfected HEK293T lysate (co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))SecondarySecondary antibody conjugated to horseradish peroxidase at 1/3000 dilution
ab122698, at 1/500 dilution, staining FAM45A in Paraffin-embedded Human pancreas tissue by Immunohistochemistry