All lanes : Anti-FAM50A antibody [EPR12816] (ab186410) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : Hela cell lysateLane 3 : 293 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-FAM50A antibody [EPR12816] (ab186410) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH-3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FAM50A with ab186410 at 1/500 and ready to use HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Immunocytochemical analysis of Hela cells fixed in 4% paraformaldehyde labling FAM50A with ab186410 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution