All lanes : Anti-FAM96B antibody [EPR10178] (ab166607) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166607 and HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.
Flow Cytometrical analysis of permeabilized K562 cells with ab166607 antibody at 1/100 (red) compared to a rabbit IgG negative (green).