Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FAN1 with ab95171 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-FAN1 antibody (ab95171) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg
3µg ab95171 were used to immunoprecipitate MTMR15 from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab95171 at 1µg/ml.Detection: chemiluminescence with exposure time of 10 seconds.