Anti-Fascin antibody [IM20]
| Name | Anti-Fascin antibody [IM20] |
|---|---|
| Supplier | Abcam |
| Catalog | ab49815 |
| Prices | $390.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | IM20 |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Prokaryotic recombinant protein corresponding to the C-terminal region of the fascin molecule |
| Description | Mouse Monoclonal |
| Gene | FSCN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab49815 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab49815 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical staining of human ovarian carcinoma tissue sections for Fascin (ab49815 at 1/100). As secondary antibody goat anti mouse biotin conjugated was use. Please see accompanying abreview for additional information.See Abreview
Immunohistochemical staining of Human Hodgkin's lymphoma (Paraffin section) for Fascin using ab49815. Note intense cytoplasmic and membrane staining of a proportion of Reed Sternberg cells.
Immunohistochemical analysis of Human oral squamous cell carcinoma tissue, staining Fascin (green) with ab49815. Tissue was incubated with primary antibody (1/100) and an AlexaFluor®488-conjugated anti-mouse IgG (1/200) was used as the secondary antibody.
Product References
Proteomic signatures of acquired letrozole resistance in breast cancer: - Proteomic signatures of acquired letrozole resistance in breast cancer:
Tilghman SL, Townley I, Zhong Q, Carriere PP, Zou J, Llopis SD, Preyan LC, Williams CC, Skripnikova E, Bratton MR, Zhang Q, Wang G. Mol Cell Proteomics. 2013 Sep;12(9):2440-55.
Fascin overexpression promotes neoplastic progression in oral squamous cell - Fascin overexpression promotes neoplastic progression in oral squamous cell
Alam H, Bhate AV, Gangadaran P, Sawant SS, Salot S, Sehgal L, Dange PP, Chaukar DA, D'cruz AK, Kannanl S, Gude R, Kane S, Dalal SN, Vaidya MM. BMC Cancer. 2012 Jan 20;12:32.
Loss of keratins 8 and 18 leads to alterations in alpha6beta4-integrin-mediated - Loss of keratins 8 and 18 leads to alterations in alpha6beta4-integrin-mediated
Alam H, Kundu ST, Dalal SN, Vaidya MM. J Cell Sci. 2011 Jun 15;124(Pt 12):2096-106.
miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 - miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1
Chiyomaru T, Enokida H, Tatarano S, Kawahara K, Uchida Y, Nishiyama K, Fujimura L, Kikkawa N, Seki N, Nakagawa M. Br J Cancer. 2010 Mar 2;102(5):883-91.