Anti-Fatty Acid Synthase antibody [EPR7465]

• Supplier: Abcam
• Catalog: ab128856
• Prices: $395.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7465
• Applications: WB FC ICC/IF ICC/IF IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide from a region of Human Fatty Acid Synthase
• Description: Rabbit Monoclonal
• Gene: FASN
• Conjugate: Unconjugated

Product images

Overlay histogram showing A549 cells stained with ab128856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128856, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : A549 cell lysateLane 4 : SHSY-5Y cell lysateLane 5 : MOLT4 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab128856 at a 1/250 dilution staining Fatty Acid Synthase in A549 cells by immunofluorescence.

ab128856 staining Fatty Acid Synthase in human peritoneal tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 2 hours. An Alexa Fluor® 647-conjugated anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.See Abreview

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD