Anti-Fatty Acid Synthase antibody [EPR7466]
| Name | Anti-Fatty Acid Synthase antibody [EPR7466] |
|---|---|
| Supplier | Abcam |
| Catalog | ab128870 |
| Prices | $388.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR7466 |
| Applications | WB IP IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues at the C terminus of Human Fatty Acid Synthase |
| Description | Rabbit Monoclonal |
| Gene | FASN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing A549 cells stained with ab128870 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128870, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870) at 1/10000 dilutionLane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : A549 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/17792_Fatty-Acid-Synthase-Primary-antibodies-ab128870-2.jpg)
All lanes : Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870) at 1/10000 dilutionLane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : A549 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
ab128870, at a 1/100 dilution, staining Fatty Acid Synthase in paraffin embedded Human liver tissue by Immunohistochemistry.
ab128870, at a 1/250 dilution, staining Fatty Acid Synthase in A549 cells by Immunofluorescence.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD
Product References
ATF4 protein deficiency protects against high fructose-induced - ATF4 protein deficiency protects against high fructose-induced
Xiao G, Zhang T, Yu S, Lee S, Calabuig-Navarro V, Yamauchi J, Ringquist S, Dong HH. J Biol Chem. 2013 Aug 30;288(35):25350-61.
Resveratrol and EGCG bind directly and distinctively to miR-33a and miR-122 and - Resveratrol and EGCG bind directly and distinctively to miR-33a and miR-122 and
Baselga-Escudero L, Blade C, Ribas-Latre A, Casanova E, Suarez M, Torres JL, Salvado MJ, Arola L, Arola-Arnal A. Nucleic Acids Res. 2014 Jan;42(2):882-92.