Anti-Fbx32 antibody
| Name | Anti-Fbx32 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab74023 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human, Pig, Chicken, Bovine, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Fbx32 |
| Description | Rabbit Polyclonal |
| Gene | FBXO32 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Fbx32 antibody (ab74023) at 2 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate Lane 3 : Skeletal Muscle (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ab74023 (1/2000) staining Fbx32 in paraffin-embedded Human heart tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit conjugated to HRP. For further experimental details please refer to abreview.See Abreview
ab74023 staining Fbx32 in rat muscle tissue sections by Immunohistochemistry (Frozen section). Tissue was fixed with 4% PFA overnight. Samples were then blocked with 10% donkey serum for 1 hour at 24°C followed by incubation with ab74023 at a 1/200 dilution, for 24 hours at 4°C. A pig anti-rabbit Alexa Fluoro 488 conjugate was used as secondary antibody at a 1/1000 dilution. Nuclei were stained using the standard Hoestch method and are depicted in blue.See Abreview
ab74023 staining cultured rat astrocytes by ICC/IF. The cultured astrocytes were fixed with paraformaldehyde and blocked with 10% donkey serum for 30 minutes at 24°C. The cultured astrocytes were then stained with ab74023 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4.5h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. FBX32 expressed in the newly born astrocytes but does not co-localise with mature astrocytes.See Abreview