All lanes : Anti-FBXO21 antibody [2B8] (ab119862) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY FBXO21 Lysates/proteins at 5 µg per lane.
All lanes : Anti-FBXO21 antibody [2B8] (ab119862) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A459 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab119862, at 1/150, staining FBXO21 in paraffin embedded Human kidney tissue by Immunohistochemistry.
ab119862, at 1/150, staining FBXO21 in paraffin embedded Human ovary tissue by Immunohistochemistry.
ab119862, at 1/100, immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FBXO21.
HEK293T cells transfected with either FBXO21 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119862, at 1/100 and then analyzed by flow cytometry.
Flow cytometric analysis of Jurkat cells, using ab119862, at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).