All lanes : Anti-FBXO21 antibody [2C7] (ab119762) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY FBXO21 cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-FBXO21 antibody [2C7] (ab119762) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab119762 at 1/150 dilution, staining FBXO21 in paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
ab119762 at 1/150 dilution, staining FBXO21 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
ab119762 at 1/150 dilution, staining FBXO21 in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.
ab119762 at 1/150 dilution, staining FBXO21 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
ab119762 at 1/150 dilution, staining FBXO21 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
ab119762 at 1/150 dilution, staining FBXO21 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
ab119762, at 1/100 dilution, staining FBXO21 in COS7 cells transiently transfected with pCMV6-ENTRY FBXO21 by Immunofluorescence.
HEK293T cells transfected with either a FBXO21 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119762 at 1/100 dilution and then analyzed by flow cytometry.
Flow cytometric analysis of HeLa cells, using ab119762 at 1/100 dilution (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometric analysis of Jurkat cells, using ab119762 at 1/100 dilution (Red), compared to a nonspecific negative control antibody (Blue).