All lanes : Anti-FBXO24 antibody [EPR13444] - C-terminal (ab181199) at 1/20000 dilutionLane 1 : Jurkat cell lysateLane 2 : MCF7 cell lysateLane 3 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling FBXO24 with ab181199 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Conterstained with Dapi blue.
Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling FBXO24 with ab181199 at 1/380 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FBXO24 with ab181199 at a 1/250 dilution. Secondary antibody: HRP cojugated Rabbit IgG prediluted. Counter stain Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling FBXO24 with ab181199 at a 1/250 dilution. Secondary antibody: HRP cojugated Rabbit IgG prediluted. Counter stain Hematoxylin.