β2-microglobulin (D8P1H) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12851
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D8P1H
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Human, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp79 of human β2-microglobulin protein.
• Description: Rabbit Monoclonal
• Gene: B2M

Product images

Western blot analysis of extracts from various cell lines using β2-microglobulin (D8P1H) Rabbit mAb (upper) and β-Actin (DA8) Rabbit mAb #8457 (lower). DLD-1 and Daudi cell lines are negative for β2-microglobulin due to genomic deletions at the β2-microglobulin locus.

Immunohistochemical analysis of paraffin-embedded HeLa (left) and DLD-1 (right) cell pellets using β2-microglobulin (D8P1H) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded colon carcinoma using β2-microglobulin (D8P1H) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human skin using β2-microglobulin (D8P1H) Rabbit mAb.

Confocal immunofluorescent analysis of PANC-1 (positive, left) and DLD-1 (negative, right) cells, using β2-microglobulin (D8P1H) Rabbit mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of DLD-1 cells (blue) and HeLa cells (green) using β2-microglobulin (D8P1H) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) #4414 was used as a secondary antibody.