Anti-FER antibody [EP1842Y]
| Name | Anti-FER antibody [EP1842Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52479 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1842Y |
| Applications | WB IP FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues on the N terminus of FER (Human) |
| Description | Rabbit Monoclonal |
| Gene | FER |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-FER antibody [EP1842Y] (ab52479) at 1/5000 dilution + Jurkat cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18438_ab52479.jpg)
Anti-FER antibody [EP1842Y] (ab52479) at 1/5000 dilution + Jurkat cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution
Overlay histogram showing Jurkat cells stained with ab52479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52479, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![FER was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to FER and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52479.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 93kDa; FER](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18440_FER-Primary-antibodies-ab52479-2.jpg)
FER was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to FER and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52479.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 93kDa; FER
Product References
Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in - Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in
McGinnis LK, Hong X, Christenson LK, Kinsey WH. Mol Reprod Dev. 2011 Jan;78(1):33-47.
Transient and bilateral increase in Neuropilin-1, Fer kinase and collapsin - Transient and bilateral increase in Neuropilin-1, Fer kinase and collapsin
Whitehead SN, Gangaraju S, Slinn J, Hou ST. Brain Res. 2010 Jul 16;1344:209-16.
The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor - The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor
Fei F, Kweon SM, Haataja L, De Sepulveda P, Groffen J, Heisterkamp N. BMC Biochem. 2010 Dec 1;11:48.