Anti-FGF21 antibody
| Name | Anti-FGF21 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66564 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | Synthetic peptide derived from within residues 1 - 100 of Human FGF21 |
| Description | Rabbit Polyclonal |
| Gene | FGF21 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-FGF21 antibody (ab66564) at 1 µg/ml + Human FGF21 full length protein (ab54141) at 0.1 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
IHC image of FGF21 staining in Human Liver Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66564, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.