Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (purified) + Mouse spleen tissue lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Rat spleen tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Human fetal liver tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (purified) + HeLa cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Immunohistochemical staining of paraffin embedded human stomach with purified ab171941 at a working dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
All lanes : Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (unpurified)Lane 1 : Human fetal liver tissue lysateLane 2 : Hela cell line lysateLane 3 : A549 cell line lysateLysates/proteins at 10 µg per lane.developed using the ECL technique
Immunohistochemical analysis of paraffin embedded Human lipoma tissue labeling FGF21 with unpurified ab171941 at 1/100 .
Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling FGF21 with unpurified ab171941 at 1/100 .