![Overlay histogram showing A549 cells stained with ab130114 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130114, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18889_ab130114-3-ab130114FC.jpg)
Overlay histogram showing A549 cells stained with ab130114 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130114, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-FGFR4 antibody [4H2B10B2] (ab130114) at 1/500 dilutionLane 1 : HEK293T cell lysate, transfected with the pCMV6-ENTRY controlLane 2 : HEK293T cell lysate, transfected with the pCMV6-ENTRY FGFR4 cDNA](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18890_FGFR4-Primary-antibodies-ab130114-1.jpg)
All lanes : Anti-FGFR4 antibody [4H2B10B2] (ab130114) at 1/500 dilutionLane 1 : HEK293T cell lysate, transfected with the pCMV6-ENTRY controlLane 2 : HEK293T cell lysate, transfected with the pCMV6-ENTRY FGFR4 cDNA
ab130114, at 1/200 dilution, staining FGFR4 in paraffin-embedded Human liver tissue by Immunohistochemistry.