All lanes : Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : Rat muscle lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescence analysis of A673 cells labeling FHL3 with ab180509 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/250 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling FHL3 with ab180509 at 1/500 dilution. Counter stain: Hematoxylin
Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/5000 dilution + Human skeletal muscle lysate at 20 µgSecondaryGoat Anti-Rabbit IgG HRP (ab136636) at 1/500 dilution