Anti-Fibrillarin antibody [38F3]

• Supplier: Abcam
• Catalog: ab4566
• Prices: $401.00
• Sizes: 250 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 38F3
• Applications: FC IHC-P ICC/IF ICC/IF IHC-F WB ICC/IF
• Species Reactivities: Mouse, Rat, Chicken, Human, Arabidopsis thaliana, C. elegans, Drosophila, S. pombe, Zebrafish, Monkey, Opossum, Plant
• Antigen: Tissue, cells or virus corresponding to Saccharomyces cerevisiae Fibrillarin
• Description: Mouse Monoclonal
• Gene: Fib
• Conjugate: Unconjugated

Product images

Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixation and staining procedure (in protocol section).

Human neuroblastoma line SH-SY5Y stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using standard fixation and staining procedure (in protocol section).

High magnification view of human Hek293 cell nuclei stained with mouse monoclonal to fibrillarin (green), counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixation and staining procedure (in protocol section).

All lanes : Anti-Fibrillarin antibody [38F3] (ab4566) at 1/2000 dilution (+ blocking buffer 1:1 in PBS + 0.1% Tween for 1 hour)Lane 1 : Lysate of the cytoplasmic protein fraction of HeLa cells Lane 2 : Lysate of the nuclear protein fraction of HeLa cellsLysates/proteins at 20 µg per lane.SecondaryAn Alexa Fluor®680-conjugated Goat anti-mouse polyclonal at 1/10000 dilutionPerformed under reducing conditions.Observed band size : 34 kDa (why is the actual band size different from the predicted?)This image is courtesy of an Abreview submitted by Svetlana KhoronenkovaSee Abreview

Overlay histogram showing HEK293 cells stained with ab4566 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4566, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Image courtesy of an anonymous Abreview.See Abreview

Product References

The transcription factor EGR1 localizes to the nucleolus and is linked to - The transcription factor EGR1 localizes to the nucleolus and is linked to

Ponti D, Bellenchi GC, Puca R, Bastianelli D, Maroder M, Ragona G, Roussel P, Thiry M, Mercola D, Calogero A. PLoS One. 2014 May 1;9(5):e96037.

A novel mechanism inducing genome instability in Kaposi's sarcoma-associated - A novel mechanism inducing genome instability in Kaposi's sarcoma-associated

Jackson BR, Noerenberg M, Whitehouse A. PLoS Pathog. 2014 May 1;10(5):e1004098.

Phosphorylation of nucleoporin Tpr governs its differential localization and is - Phosphorylation of nucleoporin Tpr governs its differential localization and is

Rajanala K, Sarkar A, Jhingan GD, Priyadarshini R, Jalan M, Sengupta S, Nandicoori VK. J Cell Sci. 2014 Aug 15;127(Pt 16):3505-20.

Def functions as a cell autonomous factor in organogenesis of digestive organs in - Def functions as a cell autonomous factor in organogenesis of digestive organs in

Tao T, Shi H, Huang D, Peng J. PLoS One. 2013 Apr 12;8(4):e58858.

Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I - Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I

Yildirim S, Castano E, Sobol M, Philimonenko VV, Dzijak R, Venit T, Hozak P. J Cell Sci. 2013 Jun 15;126(Pt 12):2730-9.

Arabidopsis DEAD-box RNA helicase UAP56 interacts with both RNA and DNA as well - Arabidopsis DEAD-box RNA helicase UAP56 interacts with both RNA and DNA as well

Kammel C, Thomaier M, Sorensen BB, Schubert T, Langst G, Grasser M, Grasser KD. PLoS One. 2013;8(3):e60644.

Developmental regulation of nucleolus size during Drosophila eye differentiation. - Developmental regulation of nucleolus size during Drosophila eye differentiation.

Baker NE. PLoS One. 2013;8(3):e58266.

MicroRNA miR-308 regulates dMyc through a negative feedback loop in Drosophila. - MicroRNA miR-308 regulates dMyc through a negative feedback loop in Drosophila.

Daneshvar K, Nath S, Khan A, Shover W, Richardson C, Goodliffe JM. Biol Open. 2013 Jan 15;2(1):1-9.

Endogenous human MDM2-C is highly expressed in human cancers and functions as a - Endogenous human MDM2-C is highly expressed in human cancers and functions as a

Okoro DR, Arva N, Gao C, Polotskaia A, Puente C, Rosso M, Bargonetti J. PLoS One. 2013 Oct 11;8(10):e77643.