Anti-Fibronectin antibody [A17]

• Supplier: Abcam
• Catalog: ab26245
• Prices: $395.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: A17
• Applications: IP IHC-P WB ELISA IHC-F ICC/IF ICC/IF
• Species Reactivities: Chicken, Bovine, Human
• Antigen: Tissue/ cell preparation (Cow) - Lysed bovine corneal endothelial cells and extracellular matrix
• Description: Mouse Monoclonal
• Gene: FN1
• Conjugate: Unconjugated

Product images

ab26245 staining Fibronectin in Human MCF-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% BSA for 1 hours at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 5% BSA) for 18 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody. Blue - DAPI, red - rhodamine phalloidin.See Abreview

ab26245 staining fibronectin in bovine cumulus-oocyte complexes  by ICC/IF (immunocytochemistry/immunofluorescence). Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 0.1% serum for 30 minutes at 37°C. The primary antibody (1/100 in 10% goat serum in polylvinylpyrrolidone) was incubated with the sample for 1 hour at 37°C. An FITC-conjugated goat anti-mouse polyclonal (1/100) was used as the secondary.  Chromatin was stained red by using the nuclear DNA stain propidium iodide.See Abreview

Product References

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