All lanes : Anti-FIGNL1 antibody (ab70841) at 1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µgLane 5 : Whole cell lysate from NIH3T3 cells at 50 µgdeveloped using the ECL technique
Detection of Human FIGNL1 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded), using ab70841 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using 1 µg/mg ab70841 and chemiluminescence with an exposure time of 30 seconds.