All lanes : Anti-FKBP25 antibody (ab168772) at 1/500 dilutionLane 1 : FKBP25-transfected 293T cell line lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique
Anti-FKBP25 antibody (ab168772) at 1/500 dilution + Human liver tissue lysate at 50 µgdeveloped using the ECL technique
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FKBP25 using purified ab168772 at 10 µg/ml. Antibody purification with Protein A will be needed prior to use.