Anti-FKBP52 antibody (ab97306) at 1/5000 dilution + H1299 whole cell lysate at 30 µg
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab97306 at 1/200 dilution (1) and merged with DNA probe (2).
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab97306 at 1/500 dilution.
Anti-FKBP52 antibody (ab97306) at 1/3000 dilution + Active human FKBP52 full length protein (ab84189) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds