All lanes : Anti-FLRT3 antibody (ab97267) at 1 µg/mlLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 3 : Lung (Human) Tissue Lysate Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 5 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 6 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab97267 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.