ab52648 staining Flt3 ligand in Rhesus monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in 20% Normanl Goat serum in 1x TTBS) for 12 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
Anti-Flt3 ligand antibody [EP1140Y] (ab52648) at 1/100000 dilution + Recombinant Flr3 ligand at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution
Immunohistochemical staining of paraffin-embedded human tonsils using ab52648 at a 1/50 dilution.Immunohistochemical staining of paraffin-embedded human tonsils using ab52648 at a 1/50 dilution.