Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122712 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122712, at 1/50 dilution, staining FLYWCH2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
All lanes : Anti-FLYWCH2 antibody (ab122712) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : FLYWCH2 over-expression HEK293T lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)