All lanes : Anti-FMN1 antibody (ab68058) at 1/500 dilutionLane 1 : FMN1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionObserved band size : 85 kDa (why is the actual band size different from the predicted?)
ab68058 staining FMN1 in mouse embryonic fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed using 4% paraformaldehyde, permeabilised in 1% Triton X-100 and then blocked using 10% horse serum + 5% BSA for 1 hour at room temperature. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).See Abreview