Anti-FMRP (phospho S499) antibody
| Name | Anti-FMRP (phospho S499) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab48127 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human, Zebrafish, Mouse, Rat |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human FMRP |
| Blocking Peptide | Human FMRP (phospho S499) peptide |
| Description | Rabbit Polyclonal |
| Gene | FMR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-FMRP (phospho S499) antibody (ab48127) at 2 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Mouse Brain Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of FMRP (phospho S499) staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 48127, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-FMRP (phospho S499) antibody (ab48127) at 0.25 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate at 20 µgLane 3 : Zebrafish heart homogenate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Subcellular fractionation and localization studies reveal a direct interaction of - Subcellular fractionation and localization studies reveal a direct interaction of
Taha MS, Nouri K, Milroy LG, Moll JM, Herrmann C, Brunsveld L, Piekorz RP, Ahmadian MR. PLoS One. 2014 Mar 21;9(3):e91465.
Deletion of PTEN produces deficits in conditioned fear and increases fragile X - Deletion of PTEN produces deficits in conditioned fear and increases fragile X
Lugo JN, Smith GD, Morrison JB, White J. Learn Mem. 2013 Nov 15;20(12):670-3.
In vivo neuronal function of the fragile X mental retardation protein is - In vivo neuronal function of the fragile X mental retardation protein is
Coffee RL Jr, Williamson AJ, Adkins CM, Gray MC, Page TL, Broadie K. Hum Mol Genet. 2012 Feb 15;21(4):900-15.
Evidence for a fragile X mental retardation protein-mediated translational switch - Evidence for a fragile X mental retardation protein-mediated translational switch
Niere F, Wilkerson JR, Huber KM. J Neurosci. 2012 Apr 25;32(17):5924-36.