Anti-FMRP antibody
| Name | Anti-FMRP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab17722 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IHC-F ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human FMRP |
| Blocking Peptide | Human FMRP peptide |
| Description | Rabbit Polyclonal |
| Gene | FMR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-MCPG sodium salt (ab120252), by ICC/IF. Decrease of FMRP expression correlates with increased concentration of (R,S)-MCPG sodium salt, as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120252 ((R,S)-MCPG sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-MCPG (ab120033), by ICC/IF. Decrease in FMRP expression correlates with increased concentration of (R,S)-MCPG, as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120033 ((R,S)-MCPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab17722 staining FMRP in SK-N-SH cells treated with (S)-MCPG (ab120059), by ICC/IF. Decrease of FMRP expression correlates with increased concentration of (S)-MCPG, as described in literature.The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120059 ((S)-MCPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
All lanes : Anti-FMRP antibody (ab17722) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
IHC image of FMRP staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17722, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ICC/IF image of ab17722 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
ab17722 staining FMRP in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.See Abreview
Anti-FMRP antibody (ab17722) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of FMRP staining in mouse frontal cortex section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was then blocked using 1% BSA for 10 mins at 21°C The section was then incubated with ab17722,1/1500 , for 2 hours at 21°C. The section was then counterstained with haematoxylin. See Abreview
Anti-FMRP antibody (ab17722) at 1 µg/ml + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab17722 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![FMRP was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FMRP and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17722.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 80kDa: FMRP.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/20011_FMRP-Primary-antibodies-ab17722-71.jpg)
FMRP was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FMRP and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17722.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 80kDa: FMRP.
ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-3,5-DHPG (ab120020), by ICC/IF. Increase in FMRP expression correlates with increased concentration of (R,S)-3,5-DHPG, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of ab120020 ((R,S)-3,5-DHPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Product References
Subcellular fractionation and localization studies reveal a direct interaction of - Subcellular fractionation and localization studies reveal a direct interaction of
Taha MS, Nouri K, Milroy LG, Moll JM, Herrmann C, Brunsveld L, Piekorz RP, Ahmadian MR. PLoS One. 2014 Mar 21;9(3):e91465.
FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity. - FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.
Bartley CM, O'Keefe RA, Bordey A. PLoS One. 2014 May 7;9(5):e96956.
Cerebral protein synthesis in a knockin mouse model of the fragile X premutation. - Cerebral protein synthesis in a knockin mouse model of the fragile X premutation.
Qin M, Huang T, Liu Z, Kader M, Burlin T, Xia Z, Zeidler Z, Hukema RK, Smith CB. ASN Neuro. 2014 Sep 23;6(5). pii: 1759091414551957. doi:
Dampened dopamine-mediated neuromodulation in prefrontal cortex of fragile X - Dampened dopamine-mediated neuromodulation in prefrontal cortex of fragile X
Paul K, Venkitaramani DV, Cox CL. J Physiol. 2013 Feb 15;591(Pt 4):1133-43.
FMRP and myelin protein expression in oligodendrocytes. - FMRP and myelin protein expression in oligodendrocytes.
Giampetruzzi A, Carson JH, Barbarese E. Mol Cell Neurosci. 2013 Sep;56:333-41.
Ovarian abnormalities in a mouse model of fragile X primary ovarian - Ovarian abnormalities in a mouse model of fragile X primary ovarian
Hoffman GE, Le WW, Entezam A, Otsuka N, Tong ZB, Nelson L, Flaws JA, McDonald JH, Jafar S, Usdin K. J Histochem Cytochem. 2012 Jun;60(6):439-56.
FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and - FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and
Darnell JC, Van Driesche SJ, Zhang C, Hung KY, Mele A, Fraser CE, Stone EF, Chen C, Fak JJ, Chi SW, Licatalosi DD, Richter JD, Darnell RB. Cell. 2011 Jul 22;146(2):247-61.
Regulation of synaptic structure and function by FMRP-associated microRNAs - Regulation of synaptic structure and function by FMRP-associated microRNAs
Edbauer D, Neilson JR, Foster KA, Wang CF, Seeburg DP, Batterton MN, Tada T, Dolan BM, Sharp PA, Sheng M. Neuron. 2010 Feb 11;65(3):373-84.
Fragile X protein family member FXR1P is regulated by microRNAs. - Fragile X protein family member FXR1P is regulated by microRNAs.
Cheever A, Blackwell E, Ceman S. RNA. 2010 Aug;16(8):1530-9.
Altered neuroligin expression is involved in social deficits in a mouse model of - Altered neuroligin expression is involved in social deficits in a mouse model of
Dahlhaus R, El-Husseini A. Behav Brain Res. 2010 Mar 17;208(1):96-105.