Anti-Fos B antibody [83B1138]
| Name | Anti-Fos B antibody [83B1138] |
|---|---|
| Supplier | Abcam |
| Catalog | ab11959 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 83B1138 |
| Applications | FC IHC-F WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | This antibody was raised against a synthetic peptide corresponding to human Fos B |
| Description | Mouse Monoclonal |
| Gene | FOSB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.See Abreview
ab11959 Fos B antibody (1/1000) immunostaining for Fos B in rat brain cortex. Staining was also detected in the striatum as expected (not shown). Rats were treated with high dose amphetamine (5mg/kg) 30 min prior to transcardial perfusion to induce Fos B expression. The staining procedure was perfomed according to the TH staining in Carlsson et al. (2005) Brain 128:559-569 with the exception that the quenching step was excluded from the staining.
![Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/20192_Fos-B-Primary-antibodies-ab11959-1.jpg)
Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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FOSB proteins in the orbitofrontal and dorsolateral prefrontal cortices of human - FOSB proteins in the orbitofrontal and dorsolateral prefrontal cortices of human
Watanabe H, Henriksson R, Ohnishi YN, Ohnishi YH, Harper C, Sheedy D, Garrick T, Nyberg F, Nestler EJ, Bakalkin G, Yakovleva T. Addict Biol. 2009 Jul;14(3):294-7.
Recurrent hypoglycemia alters hypothalamic expression of the regulatory proteins - Recurrent hypoglycemia alters hypothalamic expression of the regulatory proteins
Al-Noori S, Sanders NM, Taborsky GJ Jr, Wilkinson CW, Zavosh A, West C, Sanders CM, Figlewicz DP. Am J Physiol Regul Integr Comp Physiol. 2008 Nov;295(5):R1446-54. doi: