Anti-FOXA2 antibody [EPR4465]

• Supplier: Abcam
• Catalog: ab108396
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4465
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Human, Mouse, Rat
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FOXA2
• Blocking Peptide: FOXA2 peptide
• Description: Rabbit Monoclonal
• Gene: FOXA2
• Conjugate: Unconjugated

Product images

Immunofluorescence staining of HepG2 cells with purified ab108396 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab108396 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

Anti-FOXA2 antibody [EPR4465] (ab108396) at 1/1000 dilution (purified) + HepG2 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-FOXA2 antibody [EPR4465] (ab108396) at 1/5000 dilution (purified) + SW480 cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-FOXA2 antibody [EPR4465] (ab108396) at 1/1000 dilutionLane 1 : HepG2 lysateLane 2 : Human colon cancer lysateLysates/proteins at 10 µg per lane.