Anti-FOXA2 antibody [EPR4466]

• Supplier: Abcam
• Catalog: ab108422
• Prices: $381.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4466
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FOXA2 aa 350-450
• Description: Rabbit Monoclonal
• Gene: FOXA2
• Conjugate: Unconjugated

Product images

Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/10000 dilution (purified) + Human colon cancer tissue lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/2000 dilution (purified) + Mouse lung tissue lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/1000 dilution (unpurified)Lane 1 : Human fetal colon tissue lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 2000

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with unpurified ab108422 at a 1/250 dilution.

Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling FOXA2 with purified ab108422 at 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.-ve control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).-ve control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

Product References

Uterine epithelial cell proliferation and endometrial hyperplasia: evidence from - Uterine epithelial cell proliferation and endometrial hyperplasia: evidence from

Gao Y, Li S, Li Q. Mol Hum Reprod. 2014 Aug;20(8):776-86.