![Overlay histogram showing Jurkat cells stained with ab107248 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107248, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/20348_ab107248-4-ab107248FC.jpg)
Overlay histogram showing Jurkat cells stained with ab107248 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107248, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-FOXD3 antibody [5G9] (ab107248) at 1/500 dilutionLane 1 : NTERA-2 cell lysateLane 2 : HUVE-12 cell lysateLane 3 : HEK293 cell lysateLane 4 : Hela cell lysateLane 5 : Jurkat cell lysateLane 6 : K562 cell lysateLane 7 : RAW264.7 cell lysateLane 8 : NIH3T3 cell lysateLane 9 : COS7 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/20349_FOXD3-Primary-antibodies-ab107248-1.jpg)
All lanes : Anti-FOXD3 antibody [5G9] (ab107248) at 1/500 dilutionLane 1 : NTERA-2 cell lysateLane 2 : HUVE-12 cell lysateLane 3 : HEK293 cell lysateLane 4 : Hela cell lysateLane 5 : Jurkat cell lysateLane 6 : K562 cell lysateLane 7 : RAW264.7 cell lysateLane 8 : NIH3T3 cell lysateLane 9 : COS7 cell lysate
Immunohistochemical analysis of FOXD3 in paraffin embedded Human lung cancer tissue, using ab107248 at a dilution of 1/200 with DAB staining.
Immunohistochemical analysis of FOXD3 in paraffin embedded Human ovarian cancer tissue, using ab107248 at a dilution of 1/200 with DAB staining.