Anti-FOXK1 antibody - ChIP Grade
| Name | Anti-FOXK1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab18196 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ChIP IP ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FOXK1 |
| Description | Rabbit Polyclonal |
| Gene | FOXK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-FOXK1 antibody - ChIP Grade (ab18196) at 1/5000 dilutionLane 1 : Human H1299 cell lysateLane 2 : Mouse embryonic stem cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 100 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview
Image courtesy of Human Protein Atlas ab18196 staining in human rectum with colo-rectal cancer, showing staining of the tumour cells (in brown). Paraffin embedded rectal tissue was incubated with ab18196 (1:1000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18196 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
All lanes : Anti-FOXK1 antibody - ChIP Grade (ab18196) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 7 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 95 kDa (why is the actual band size different from the predicted?)Exposure time : 5 minutes
ICC/IF image of ab18196 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18196, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![FOXK1 - ChIP Grade was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FOXK1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18196.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 96kDa:FOXK1.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/20450_FOXK1-Primary-antibodies-ab18196-7.jpg)
FOXK1 - ChIP Grade was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FOXK1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18196.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 96kDa:FOXK1.
Product References
Dissection of the C-terminal region of E1A redefines the roles of CtBP and other - Dissection of the C-terminal region of E1A redefines the roles of CtBP and other
Cohen MJ, Yousef AF, Massimi P, Fonseca GJ, Todorovic B, Pelka P, Turnell AS, Banks L, Mymryk JS. J Virol. 2013 Sep;87(18):10348-55.
Adenovirus type 5 E1A and E6 proteins of low-risk cutaneous beta-human - Adenovirus type 5 E1A and E6 proteins of low-risk cutaneous beta-human
Komorek J, Kuppuswamy M, Subramanian T, Vijayalingam S, Lomonosova E, Zhao LJ, Mymryk JS, Schmitt K, Chinnadurai G. J Virol. 2010 Mar;84(6):2719-31.
Functional interactions between the Forkhead transcription factor FOXK1 and the - Functional interactions between the Forkhead transcription factor FOXK1 and the
Freddie CT, Ji Z, Marais A, Sharrocks AD. Nucleic Acids Res. 2007;35(15):5203-12. Epub 2007 Aug 1.