Anti-FOXO1A antibody

• Supplier: Abcam
• Catalog: ab12161
• Prices: $382.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Human, Chicken, Pig
• Antigen: Synthetic peptide: LPNQSFPHSVKTTTHS conjugated to KLH, corresponding to amino acids 636-651 of Human FOXO1A
• Description: Rabbit Polyclonal
• Gene: FOXO1
• Conjugate: Unconjugated

Product images

ab12161 staining FOXO1A (1/200) in mouse E11 cortical cells cultured for 6 days. Secondary antibody Alexa 546 goat anti rabbit IgG 1/1000 was incubated for 2 hours at room temperature. Images were taken using a Zeiss camera under 40X objectives.

ab12161 staining FOXOA1 (1/200) in the cortical plate (CP) and ventricular zone (VZ=CNS germinal zone) of mouse E13 forebrain tissue. E13 mouse embryos were fixed in 4% paraformaldehyde in PB overnight at 4°C then transferred to 30% sucrose in PBS.  Brains were frozen in TissueTek and stored at -80°C. Cryostat coronal sections were cut at 12µm. Sections were blocked in 0.1% triton in PBS containing 10% normal serum. Primary antibodies were incubated on the sections at 4°C overnight.  Secondary antibody Alexa 546 goat anti rabbit IgG 1/1000 was incubated for 2 hours at room temperature. Images were taken using a Zeiss camera under 40X objectives.

All lanes : Anti-FOXO1A antibody (ab12161) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryRabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Product References

Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional - Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional

Picard M, Zhang J, Hancock S, Derbeneva O, Golhar R, Golik P, O'Hearn S, Levy S, Potluri P, Lvova M, Davila A, Lin CS, Perin JC, Rappaport EF, Hakonarson H, Trounce IA, Procaccio V, Wallace DC. Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):E4033-42. doi:

AMPK and FoxO1 regulate catalase expression in hypoxic pulmonary arterial smooth - AMPK and FoxO1 regulate catalase expression in hypoxic pulmonary arterial smooth

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The increase in body weight induced by lack of methyl CpG binding protein-2 is - The increase in body weight induced by lack of methyl CpG binding protein-2 is

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The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy - The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy

Sundaresan NR, Vasudevan P, Zhong L, Kim G, Samant S, Parekh V, Pillai VB, Ravindra PV, Gupta M, Jeevanandam V, Cunningham JM, Deng CX, Lombard DB, Mostoslavsky R, Gupta MP. Nat Med. 2012 Nov;18(11):1643-50.

Central venous hypoxemia is a determinant of human atrial ATP-sensitive potassium - Central venous hypoxemia is a determinant of human atrial ATP-sensitive potassium

Raeis V, Philip-Couderc P, Roatti A, Habre W, Sierra J, Kalangos A, Beghetti M, Baertschi AJ. Hypertension. 2010 May;55(5):1186-92.

O-GlcNAcylation enhances FOXO4 transcriptional regulation in response to stress. - O-GlcNAcylation enhances FOXO4 transcriptional regulation in response to stress.

Ho SR, Wang K, Whisenhunt TR, Huang P, Zhu X, Kudlow JE, Paterson AJ. FEBS Lett. 2010 Jan 4;584(1):49-54.

The FoxO transcription factors and metabolic regulation. - The FoxO transcription factors and metabolic regulation.

Nakae J, Oki M, Cao Y. FEBS Lett. 2008 Jan 9;582(1):54-67. Epub 2007 Nov 20.