Anti-Foxo6 antibody
| Name | Anti-Foxo6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab48730 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat |
| Antigen | Synthetic peptide: RAVSMDNGAK FLRIKGKASK KKQLHLPERS PDDSPPGAPV PGPLSASAKW, corresponding to a region within N terminal amino acids 181-230 of Mouse Foxo6 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | Foxo6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Foxo6 antibody (ab48730) at 1.25 µg/ml + SP2/0 lysate at 10 µg with 5% skimmed milk in PBSSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab48730 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48730, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.