ab17726 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) stomach. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
All lanes : Anti-FOXP4 antibody (ab17726) at 1/1000 dilutionLane 1 : 50ug Lung epithelial cell line, non-transfected.Lane 2 : 50ug Lung epithelial cell line, transfected with FOXP4.SecondaryHRP cpnjugated goat anti-rabbitPerformed under reducing conditions.
Anti-FOXP4 antibody (ab17726) at 1 µg/ml + Human FOXP4 Partial Recombinant Protein (Tagged) at 0.1 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.