Anti-Fragilis antibody
| Name | Anti-Fragilis antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15592 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P IHC ICC/IF ICC/IF IHC-F WB |
| Species Reactivities | Mouse, Rat |
| Antigen | Synthetic peptide derived from residues 1 - 50 of Mouse Fragilis |
| Blocking Peptide | Fragilis peptide |
| Description | Rabbit Polyclonal |
| Gene | Ifitm3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Fragilis antibody (ab15592) at 1 µg/mlLane 1 : Mouse Embryonic Stem Cell lysate Lane 2 : Mouse Embryonic Germ Cell lysate Lane 3 : Mouse Embryonic Stem Cell lysate with Fragilis peptide (ab15737) at 1 µg/mlLane 4 : Mouse Embryonic Germ Cell lysate with Fragilis peptide (ab15737) at 1 µg/mlLysates/proteins at 10 µg per lane.
IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).ab15592 (anti-Fragilis) = redOct4 = greenDAPI (DNA) = blueThe dilution of ab15592 used was 10 ug/ml.
Anti-Fragilis antibody, ab15592, recognised a small proportion of cells in a culture of human embryonic stem cells differentiated into a heterogenous mix of cells. Based upon the sequence of the immunogen used to generate this antibody the protein it recognizes is likely to be IFITM2 and/or IFITM3.
Fragilis - Primordial Germ Cell Marker was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5µg of Rabbit polyclonal to Fragilis and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15592.Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.Band: 13kDa: Fragilis - Primordial Germ Cell Marker.
Product References
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IFITM3 restricts the morbidity and mortality associated with influenza. - IFITM3 restricts the morbidity and mortality associated with influenza.
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Ifitm3 limits the severity of acute influenza in mice. - Ifitm3 limits the severity of acute influenza in mice.
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IFITM3 inhibits influenza A virus infection by preventing cytosolic entry. - IFITM3 inhibits influenza A virus infection by preventing cytosolic entry.
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Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis - Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis
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