Anti-Frataxin antibody [18A5DB1]

• Supplier: Abcam
• Catalog: ab110328
• Prices: $400.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 18A5DB1
• Applications: WB ICC/IF ICC/IF FC IHC-P
• Species Reactivities: Human
• Antigen: Recombinant full length mature protein, corresponding to amino acids 56-210 of Human Frataxin
• Description: Mouse Monoclonal
• Gene: FXN
• Conjugate: Unconjugated

Product images

Anti-Frataxin antibody [18A5DB1] (ab110328) at 5 µg/ml + Recombinant Human Frataxin at 0.0005 µg

Immunocytochemistry analysis using ab110328 at 0.5µg/ml staining Frataxin in normal Human fibroblasts (MRC5) (fixed and permeabilized) followed by an AlexaFluor® 594-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml). .

Flow cytometric analysis using ab110328 at 1µg/ml staining Frataxin in HL60 cells (blue). Isotype control antibody (red).

IHC image of Frataxin staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110328, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

miR-886-3p levels are elevated in Friedreich ataxia. - miR-886-3p levels are elevated in Friedreich ataxia.

Mahishi LH, Hart RP, Lynch DR, Ratan RR. J Neurosci. 2012 Jul 4;32(27):9369-73.

Blood cells from Friedreich ataxia patients harbor frataxin deficiency without a - Blood cells from Friedreich ataxia patients harbor frataxin deficiency without a

Selak MA, Lyver E, Micklow E, Deutsch EC, Onder O, Selamoglu N, Yager C, Knight S, Carroll M, Daldal F, Dancis A, Lynch DR, Sarry JE. Mitochondrion. 2011 Mar;11(2):342-50.

The neuropathology of late-onset Friedreich's ataxia. - The neuropathology of late-onset Friedreich's ataxia.

Koeppen AH, Morral JA, McComb RD, Feustel PJ. Cerebellum. 2011 Mar;10(1):96-103.

Two new pimelic diphenylamide HDAC inhibitors induce sustained frataxin - Two new pimelic diphenylamide HDAC inhibitors induce sustained frataxin

Rai M, Soragni E, Chou CJ, Barnes G, Jones S, Rusche JR, Gottesfeld JM, Pandolfo M. PLoS One. 2010 Jan 21;5(1):e8825.

Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial - Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial

Nadanaciva S, Willis JH, Barker ML, Gharaibeh D, Capaldi RA, Marusich MF, Will Y. J Immunol Methods. 2009 Mar 31;343(1):1-12.

Pimelic diphenylamide 106 is a slow, tight-binding inhibitor of class I histone - Pimelic diphenylamide 106 is a slow, tight-binding inhibitor of class I histone

Chou CJ, Herman D, Gottesfeld JM. J Biol Chem. 2008 Dec 19;283(51):35402-9.

The in vivo mitochondrial two-step maturation of human frataxin. - The in vivo mitochondrial two-step maturation of human frataxin.

Schmucker S, Argentini M, Carelle-Calmels N, Martelli A, Puccio H. Hum Mol Genet. 2008 Nov 15;17(22):3521-31.

Mitochondrial localization of human frataxin is necessary but processing is not - Mitochondrial localization of human frataxin is necessary but processing is not

Long S, Jirku M, Ayala FJ, Lukes J. Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13468-73. doi:

Lateral-flow immunoassay for the frataxin protein in Friedreich's ataxia patients - Lateral-flow immunoassay for the frataxin protein in Friedreich's ataxia patients

Willis JH, Isaya G, Gakh O, Capaldi RA, Marusich MF. Mol Genet Metab. 2008 Aug;94(4):491-7.